![]() ![]() ![]() Molecular Probes nucleic acid–binding cyanine dyes share several important spectroscopic and physical properties: Other nucleic acid stains, including acridine orange, 7-AAD, LDS 751 and hydroxystilbamidine.Minor-groove binders ( Figure 8.1.1), such as DAPI and the Hoechst dyes.Intercalating dyes ( Figure 8.1.1), such as ethidium bromide and propidium iodide.The three classes of classic nucleic acid stains ( Properties of classic nucleic acid stains-Table 8.4) include: Amine-reactive SYBR dye that can be used to form bioconjugates.Cell-permeant SYTO™ family of dyes ( Cell-permeant cyanine nucleic acid stains-Table 8.3).Cell-impermeant TOTO™, TO-PRO™ and SYTOX™ families of dyes ( Cell membrane–impermeant cyanine nucleic acid stains-Table 8.2).Premier dyes for ultrasensitive nucleic acid quantitation and gel staining ( Specialty nucleic acid reagents for molecular biology-Table 8.1).The four classes of Invitrogen™ Molecular Probes™ cyanine dyes include: The sections that follow describe applications of these nucleic acid stains for genomics research. This section discusses the physical properties of the various classes of dyes listed below. We offer an extensive assortment of nucleic acid stains, many of which have been developed in our research laboratories. Chapter 23-Antifades and Other Tools for Fluorescence Applications.Chapter 22-Probes for Membrane Potential.Chapter 21-Indicators for Na+, K+, Cl– and Miscellaneous Ions.Chapter 19-Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions.Chapter 18-Probes for Reactive Oxygen Species, Including Nitric Oxide.Chapter 17-Probes for Signal Transduction.Chapter 16-Probes for Endocytosis, Receptors and Ion Channels.Chapter 15-Assays for Cell Viability, Proliferation and Function.Chapter 14-Fluorescent Tracers of Cell Morphology and Fluid Flow.Chapter 13-Probes for Lipids and Membranes.Chapter 11-Probes for Cytoskeletal Proteins.Chapter 10-Enzyme Substrates and Assays.Chapter 9-Protein Detection and Analysis.Chapter 8-Nucleic Acid Detection and Analysis.Chapter 7-Antibodies, Avidins and Lectins.Chapter 6-Ultrasensitive Detection Technology.Chapter 5-Crosslinking and Photoactivatable Reagents.Chapter 4-Biotin and Hapten Derivatives.Chapter 3-Click Chemistry and other Functional Group Modifications.Chapter 1-Fluorophores and Their Amine-Reactive Derivatives.P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal. Remove contact lenses, if present and easy to do. P305+P354+P338 IF IN EYES: Immediately rinse with water for several minutes. P362+P364 Take off contaminated clothing and wash it before reuse. #PHOTOFLOW MSDS SKIN#P332+P317 If skin irritation occurs: Get medical help. P302+P352 IF ON SKIN: Wash with plenty of water/. P301+P317 IF SWALLOWED: Get medical help. P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/. P270 Do not eat, drink or smoke when using this product. H411 Toxic to aquatic life with long lasting effects Poly(oxy-1,2-ethanediyl),α-ω-hydroxy- Glycols,polyethylene,mono ether Phenol,p-(1,1,3,3-tetramethylbutyl)-,monoether with polyethylene glycol Antarox A 200 (p-t-Octylphenoxy)polyethoxyethanol α-ω-hydroxypoly(oxyethylene) Polyethylene glycol p-octylphenol ether Triton X 165 Triton X 305 Polyethylene glycol mono ether Triton X 405 Triton X 100 Triton X 45 OPE 30 Polyethylene glycol mono(p-tert-octylphenyl) ether Polyethylene glycol mono(4-octylphenyl) ether Polyethylene glycol p-tert-octylphenyl ether Triton X 102 Triton X 705 Poly(oxyethylene) p-tert-octylphenyl ether Polyethylene glycol mono(4-tert-octylphenyl) ether Polyethylene glycol p-(1,1,3,3-tetramethylbutyl)phenyl ether Polyethylene glycol p-octylphenyl ether Triton X 35 Polyoxyethylene mono(octylphenyl) ether Polyoxyethylene (13) octylphenyl ether Polyoxyethylene (9) octylphenyl ether Preceptin Hydrol SW Ortho-Gynol p-tert-Octylphenoxy polyethoxyethanol NOP 90 Texofor FP 300 Koromex II Polyethylene oxide-p-tert-octylphenyl ether Polyoxyethylene glycol-p-tert-octylphenyl ether Polyethylene glycol p-tert-octylphenol ether TX 100 TX 305 TX 102 Triton X 101 Iconol OP TX 405 Triton X 15 Triton X 705-70 Triton X 114 Photo-Flow 200 Anapoe X 114 Octoxynol 5 Nonidel P 40 Triton 100 1 3 6 6 6 8 11 12 17 30 93 101 200 205 209 ![]()
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